Single neuron preparation using lasercapture microdissection.

<p>Schematic illustration (<b>A</b>) and Nissl-staining (<b>B</b>) of rat hypothalamic nuclei at plane 29 according to Paxinos and Watson <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0078799#pone.0078799-Paxinos1" target="_blank">[44]</a>; scale bar = 100 µm. Insert shows single neuron preparations from the arcuate nucleus (ARC) using Lasercapture microdissection (LMD); scale bar = 25 µm. VMN = ventromedial hypothalamic nucleus, SN = microdissected single neuron.