Localization of afadin and AJ proteins in the <i>afadin</i>-cKO cerebral aqueduct and third ventricle.

<p>The sections of the cerebral aqueduct and the third ventricle were stained with the indicated Abs in the control and <i>afadin</i>-cKO mice at E13.5 and P0. (<b>A and B</b>) The cerebral aqueduct; (<b>C and D</b>) the third ventricle; (<b>A and C</b>) at E13.5; and (<b>B and D</b>) at P0; (<b>a1 and a2</b>) with the anti-N-cadherin Ab; (<b>b1 and b2</b>) with the anti-nectin-1 Ab; and (<b>c1 and c2</b>) with the anti-afadin Ab. (<b>a1–d1</b>) The control mice; and (<b>a2–d2</b>) the <i>afadin</i>-cKO mice. Aq, aqueduct; 4 V, fourth ventricle. Scale bars: 200 µm.