TM-PUMA Decreases Clonogenic Growth in HER2 Overexpressing Cells.

<p>BT-474 cells (a, c, e) and MDA-MB-453 cells (b, d, f) were transfected with an empty vector, WT-PUMA, or TM-PUMA for 48 hrs (e, f). Transfected cells were then seeded into 6-well plates either without (a and b) or with soft agarose (c and d) and incubated at 37°C for 14–21 days. Colonies were then counted, stained with crystal violet blue, and images were taken.