<p>This construct is modified from the pT2AL200R150G plasmid <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0079127#pone.0079127-Urasaki1" target="_blank">[70]</a>. Tol2: the left and right terminal regions of the full-length Tol2, ATP1A3p: the porcine <i>ATP1A3</i> promoter sequence, GFP: Green Fluorescence Protein, Intron: the rabbit β-globin intron, polyA: SV40 polyA signal.</p
AbstractWe report here development of a novel gene trap method in zebrafish using the Tol2 transposo...
<p><b>(A)</b>. Schematic representation of the pBHSA plasmid used in our transgenic experiment. pBL ...
(A) A linear diagram of 13 unique constructs of Tmie used in our experiments. Full-length zebrafish ...
<div><p>(A) Visualization of <i>Tol2</i>-mediated somatic transposition in zebrafish (see somatic tr...
<p><b>A.</b> Diagram of the vector and conditional regulation. The gene trap cassette is flanked by ...
Transgenesis is an essential technique for any genetic model. Tol2-based transgenesis paired with Ga...
Transgenesis of large DNA constructs is essential for gene function analysis. Recently, Tol2 transpo...
Abstract Background Tol2, a member of the hAT family of transposons, has become a useful tool for ge...
The recent establishment of recombination-based cloning systems has greatly facilitated the analysis...
Here we report the generation and use of three multisite Gateway TA-cloning plasmids, p5E-TA, pME-TA...
<p>Transgenic 5(B), while a wild-type sibling does not (A). Brightfield images of the larvae depicte...
The generation of zebrafish transgenic lines that express specific fluorophores in a cell- or tissue...
<p>(A) Schematic diagram of the structure of PSGH2/MYCN recombinant plasmid. A mouse-MYCN fragment w...
Vectors to drive hACE2 expression in the zebrafish embryo : The two different vectors use pTol2zS26...
Genomic deletions induced by imprecise excision of transposons have been used to disrupt gene functi...
AbstractWe report here development of a novel gene trap method in zebrafish using the Tol2 transposo...
<p><b>(A)</b>. Schematic representation of the pBHSA plasmid used in our transgenic experiment. pBL ...
(A) A linear diagram of 13 unique constructs of Tmie used in our experiments. Full-length zebrafish ...
<div><p>(A) Visualization of <i>Tol2</i>-mediated somatic transposition in zebrafish (see somatic tr...
<p><b>A.</b> Diagram of the vector and conditional regulation. The gene trap cassette is flanked by ...
Transgenesis is an essential technique for any genetic model. Tol2-based transgenesis paired with Ga...
Transgenesis of large DNA constructs is essential for gene function analysis. Recently, Tol2 transpo...
Abstract Background Tol2, a member of the hAT family of transposons, has become a useful tool for ge...
The recent establishment of recombination-based cloning systems has greatly facilitated the analysis...
Here we report the generation and use of three multisite Gateway TA-cloning plasmids, p5E-TA, pME-TA...
<p>Transgenic 5(B), while a wild-type sibling does not (A). Brightfield images of the larvae depicte...
The generation of zebrafish transgenic lines that express specific fluorophores in a cell- or tissue...
<p>(A) Schematic diagram of the structure of PSGH2/MYCN recombinant plasmid. A mouse-MYCN fragment w...
Vectors to drive hACE2 expression in the zebrafish embryo : The two different vectors use pTol2zS26...
Genomic deletions induced by imprecise excision of transposons have been used to disrupt gene functi...
AbstractWe report here development of a novel gene trap method in zebrafish using the Tol2 transposo...
<p><b>(A)</b>. Schematic representation of the pBHSA plasmid used in our transgenic experiment. pBL ...
(A) A linear diagram of 13 unique constructs of Tmie used in our experiments. Full-length zebrafish ...