ArxE mice are able to specify a subset of α-cells at E15.5.

<p>(<b>A–F</b>): Control and ArxE E15.5 pancreatic sections were stained for glucagon (green) and insulin (red; A–B), somatostatin (Sst; red; C–D), and ghrelin (Ghr; red; E–F). Scale bar denotes 50 µm. (<b>G</b>): Quantification of total endocrine (ChrgA) and β-, δ-, and α-cell mass in control (black bar) and ArxE (white bar) pancreata. (<b>H</b>): Quantification of transcript levels for each endocrine hormone in control (black bar) and ArxE pancreata (white bar) at E15.5 using qRT-PCR. All results are graphed as fold change relative to littermate controls ± standard error of the mean. Significance is denoted with (*) when p≤0.05. All analysis consists of 4–5 animals per group.