280B is over-expressed in prostate tumors and correlates with reduced p53 protein expression.

<p>(<b>A</b>)Real-time PCR and Western blotting were used to detect the expression of 280B in normal prostate (PrEC) and prostate cancer cell lines (LNCaP, C81, and CWR-22Rv1). (<b>B</b>) Immuno-staining of 280B in 4 matched pairs of prostate tissues, normal versus tumor. (<b>C</b>) Cell extract from 4 normal prostate tissues and 9 tumor tissues were subjected to Western blotting to detect 280B, p53, and Mdm2 expression levels. β-actin served as a loading control.