De novo angiogenesis is visualized 15 days following AV loop preparation.

<p>After injection of black-appearing India-ink into the aorta cross sections of the explanted and paraffin embedded AV loop construct were obtained perpendicular to the longitudinal AV loop axis which are the central dark areas. Preparations are stained with hematoxylin and eosin (A) or without staining (B). The central AV loop vein graft is surrounded by newly formed and dye filled vessels. Micro-CT analyses following microfil© perfusion (C) confirm the strong luminal neovascularisation arising from the venous graft without the need of additional growth factors. Scale bars: 1000 µm.