Liver PKAc protein levels during freezing and thawing.

<p>(A) Representative immunoblots of hepatic PKAc protein in Alaskan and Ohioan unfrozen (U), 48 h-frozen (F), and 120 h-thawed (T) frogs (2 samples per treatment). (B) Hepatic PKAc protein amounts (mean ± SEM; <i>N</i> = 4–8) during freezing (48 h) and thawing (120 h) in Alaskan and Ohioan <i>R. sylvatica</i> as compared to that in unfrozen frogs (U). Asterisk indicates that the value differs from the mean for the corresponding sample of unfrozen frogs (Dunnett’s, <i>P</i><0.05); dagger indicates that the value differs between populations (Bonferroni, <i>P</i><0.05).