Runx3-bound regions and their corresponding annotated genes in IL-2-activated compared to resting CD8-TC and NKC.

<p>(A) Overlap of Runx3-bound (upper panels) regions or their corresponding genes (lower panels) in resting and IL-2-activated CD8-TC (left) or NKC (right). The genes corresponding to Runx3-bound regions were derived using GREAT [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0080467#B19" target="_blank">19</a>]. (B) Recruitment of Runx3 to de novo IL-2-activated regions in Gzme-Gzmc (top) and Serpinb1c-Serpinb1b (bottom) loci, in NKC and CD8-TC, respectively. Brown tracing and rectangles represent Runx3 ChIP-seq wiggle files and the positions of Runx3 peaks, respectively, as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0080467#pone-0080467-g001" target="_blank">Figure 1</a>. Note that some de novo Runx3-bound regions in Serpin genes in IL-2-activated CD8-TC appear to bind Runx3 in resting CD8-TC, but these regions are not scored by MACS as peaks in resting CD8-TC due to the higher background. (C) Overlap of Runx3-bound regions (upper panel) and their corresponding genes (lower panel) in IL-2-activated CD8-TC and NKC.