The Disheveled protein enriched at the vegetal pole is embedded in a vegetal cortical domain.

<p>Cortices were collected from S. purpuratus eggs and zygotes, prepared for anti-Dsh immunofluorescence and viewed using scanning confocal microscopy. (A-C) Wide field view of cortices isolated from unfertilized eggs shows the accumulation of Dsh in the vegetal cortical domain (—, 50µm). (D-F) A high magnification view of a single cortex labeled by anti-Dsh antibodies and phalloidin shows a uniform F-actin distribution and concentrated Dsh in one domain, (—, 10µm). Note the punctate appearance of the Dsh staining. (G-I) A cortex isolated from a zygote labeled with anti-Dsh antibodies and phalloidin shows that Dsh remains anchored in the vegetal cortical domain following fertilization (—, 10µm). (J-L) A higher magnification view of the Dsh puncta at the vegetal cortex shows that Dsh is embedded between short actin filaments, (—, 2.5µm). Insets on the left and top of each panel from D-L shows a 90˚ rotation of the 3D confocal dataset shown in D-L (x-y) and allows the same image to be viewed in cross sections, x-z and z-y, showing Dsh is embedded between short actin filaments.