The pool of Disheveled protein in the vegetal cortical domain is differentially post-translationally modified.

<p>Approximately 40 mg of total protein from (A) eggs, (B) isolated cortical fragments, (C) 16-cell stage embryos, (D) 16-cell stage micromeres, were collected from <i>S</i>. <i>purpuratus</i> and then subjected to 2D gel electrophoresis, separating the proteins first by charge (on a pH 4 to pH 7 IPG strip) then by size, and then to Western blot analysis using anti-Dsh antibodies. Dsh (red) isoforms in isolated cortex samples and micromere samples are enriched near the acidic (left) side of the gel, while whole embryo samples show the presence of Dsh protein species that are not detected in the isolated cortex and 16-cell stage micromeres. Tubulin (green) serves as an internal control.