Antibody and FcRgamma-chain deficient mice show increased larval burdens, larger granulomas, a stronger tendency to develop necrosis and higher in-tissue motility as compared to wildtype mice.

<p>Mice were challenge infected with 200 infective L3 larvae after drug cured 1° infection and sacrificed on day 4 p.i.; (A) Numbers of tissue invasive larvae were analysed with a modified Baermann apparatus. (B) Paraffin sections of the duodenum of infected mice were hematoxylin and eosin (H&E) stained, (C/D) granuloma areas were quantified in light microscopy images of the largest cross-section of serial sections with larvae (C) or without larvae (D). (E) Necrosis in H&E-stained sections was scored by a blinded pathologist. (F–H) Absolute (F) and relative (H) in-tissue motility or larval length (G) was assessed by light microscopy combined with our Fiji macro. Pooled data of two independent experiments with 5 mice per group are shown as mean + SEM (*p<0.05, **p<0.01, ***p<0.001, Mann-Whitney test).