Rng3p is required the function of non-essential myosin-II Myp2p.

<p>A Myp2p-head/Myo2p-tail chimera construct was tested for its ability to rescue the growth of temperature-sensitive <i>rng3-65</i> mutant cells under restrictive growth conditions (36°C). Plasmid transformants were grown under permissive conditions (25°C) on EMM-Leu⁻ Ura⁻ minimal media plates before re-streaking and subsequent incubation on plates at 36°C. Double drop-out plates were employed to accommodate the differing markers of the p<i>GFP-myp2-head/myo2-tail</i> (<i>LEU2</i>) and p<i>GFP-rng3</i> (<i>ura4⁺</i>) plasmids. The vector alone transformant (left, negative control) carried empty <i>LEU2</i> and <i>ura4⁺</i> plasmids; the Rng3p transformant (center, positive control) carried an empty <i>LEU2</i> vector and p<i>GFP-rng3</i>; and the Myp2p-head/Myo2p-tail transformant (right) carried p<i>GFP-myp2-head/myo2-tail</i> and an empty <i>ura4⁺</i> vector.