Survival rates and electrolyte leakages of <i>Pro_35S:ARR22:HA</i> and <i>Pro_35S:ARR22^D74N:HA</i> transgenic <i>Arabidopsis</i> plants treated with or without DEX after dehydration stress treatment.

<p>(A) Representative filters showing wild-type, <i>Pro_35S:ARR22:HA</i>, and <i>Pro_35S:ARR22^D74N:HA</i> transgenic <i>Arabidopsis</i> plants subjected to dehydration stress tolerance assays. The number indicated beside the filter is the number of plants that survived after recovery process of 25 initial seedlings. Seedlings were grown for 9 d and incubated with 10 µM DEX or mock for an additional 5 d. The DEX or mock-treated plants were dehydrated on filter paper for 3 h and 40 min and allowed to recover with 2 ml of sterile distilled-water for 3 d. Plants surviving after recovery were counted. Experiments were repeated three times. (B) Determination of survival rates after dehydration stress treatment. Experiments are described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0079248#pone-0079248-g002" target="_blank">Fig 2A</a> legend. Bar indicate standard error; n = 25. Statistically significant changes compared with plants treated without DEX are indicated by * when <i>p</i><0.05 or by ** when <i>p</i><0.01 (Student’s <i>t</i>-test), respectively. (C) Measurements of electrolyte leakage after dehydration stress treatment. Electrolyte leakage was measured for plants treated as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0079248#pone-0079248-g002" target="_blank">Figure 2B</a>. Experiments were repeated three times. Bar indicates standard error. Statistical analysis from ten assays was conducted as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0079248#pone-0079248-g002" target="_blank">Figure 2B</a>.