<p>Cultures were grown at different temperatures either in complex medium (A, C, E) or in synthetic medium with glucose (B, D, F). They were grown at the standard temperature of 42°C (A, B), at the reduced temperature of 30°C (C, D) and the elevated temperature of 50°C (E, F). Average results from triplicate cultures and their standard deviations are shown. The color code for the mutants is given in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0077188#pone-0077188-g003" target="_blank">Figure 3</a>.</p
<p>For all strain/substrate measurements, we determined the relative change in growth rate by changi...
<p>The growth of WP3 and the WP3Δorf2 mutant under different temperature and pressure conditions wer...
<p>(<i>A</i>) Relative exponential growth rates (parental strain set to 1.0) in single cultures for ...
<p>Nine gene deletion mutants and the <i>H26Δdhfr</i> wild-type were cultivated in microtiter plates...
<p>Cultures were grown at the optimal salt concentration of 2.1(A), the reduced salt concentration o...
<p>The <i>aIF2B</i> double deletion mutant (<i>HVO_1934, HVO_2706</i>) was grown in medium with six ...
<p>Growth of <i>aIF2γ</i> depletion mutant (Depl_1901, green lines) and the wild-type (black lines) ...
<p>Cells were cultivated in synthetic medium with the optimal NaCl concentration of 2.1(A), pyruvate...
<p>(A and B): Un-evolved K and L strains were grown in mono-culture in glucose minimal medium supple...
Reduced culture temperature is an increasingly popular practice to improve recombinant protein yield...
<p>(A), The effect of elevated temperature (55°C) on cellular viability. Exponentially growing SH100...
<p>a)Figures represent the relative colony size of the mutant on minimal glucose plates without hist...
<p>Serial dilutions of <i>sec9-7</i>, <i>sec9-4</i>, or wild-type cell suspensions were spotted onto...
<p>WT (<b>▪</b>) and mutant cells (<b>□</b>) were grown as described for Fig. 6. Gn times shown abov...
<p>(▪) WT WP3, (•) <i>fur</i> mutant, (▴) WT WP3 with 60 µM iron chelator, and (▾) <i>fur</i> mutant...
<p>For all strain/substrate measurements, we determined the relative change in growth rate by changi...
<p>The growth of WP3 and the WP3Δorf2 mutant under different temperature and pressure conditions wer...
<p>(<i>A</i>) Relative exponential growth rates (parental strain set to 1.0) in single cultures for ...
<p>Nine gene deletion mutants and the <i>H26Δdhfr</i> wild-type were cultivated in microtiter plates...
<p>Cultures were grown at the optimal salt concentration of 2.1(A), the reduced salt concentration o...
<p>The <i>aIF2B</i> double deletion mutant (<i>HVO_1934, HVO_2706</i>) was grown in medium with six ...
<p>Growth of <i>aIF2γ</i> depletion mutant (Depl_1901, green lines) and the wild-type (black lines) ...
<p>Cells were cultivated in synthetic medium with the optimal NaCl concentration of 2.1(A), pyruvate...
<p>(A and B): Un-evolved K and L strains were grown in mono-culture in glucose minimal medium supple...
Reduced culture temperature is an increasingly popular practice to improve recombinant protein yield...
<p>(A), The effect of elevated temperature (55°C) on cellular viability. Exponentially growing SH100...
<p>a)Figures represent the relative colony size of the mutant on minimal glucose plates without hist...
<p>Serial dilutions of <i>sec9-7</i>, <i>sec9-4</i>, or wild-type cell suspensions were spotted onto...
<p>WT (<b>▪</b>) and mutant cells (<b>□</b>) were grown as described for Fig. 6. Gn times shown abov...
<p>(▪) WT WP3, (•) <i>fur</i> mutant, (▴) WT WP3 with 60 µM iron chelator, and (▾) <i>fur</i> mutant...
<p>For all strain/substrate measurements, we determined the relative change in growth rate by changi...
<p>The growth of WP3 and the WP3Δorf2 mutant under different temperature and pressure conditions wer...
<p>(<i>A</i>) Relative exponential growth rates (parental strain set to 1.0) in single cultures for ...