Impaired glucose homeostasis in DKO mice during prolonged fasting.

<p>(A to E) Livers were isolated before (0-hr) and after fasting (24- and 48-hr). (A) Glycogen storage in the liver of WT and DKO. n = 8/group (B) Representative PAS staining of the liver of the WT and DKO mice. Scale bar = 100 µm. (C and D) The indicated metabolites from the livers of WT and DKO mice after 48-hr fast were measured using metabolome analysis. n = 7/group. (E) The expression of genes involved in gluconeogenesis was determined using quantitative real-time PCR. (F) Estimated gluconeogenesis using the pyruvate challenge test. After 24 hour of fasting, pyruvate (2 gram/kg) was intraperitoneally injected. Blood was taken from the tail vein to measure the blood glucose levels at the indicated time points. n = 6/group. Data are shown as the mean ± SE. WT vs. DKO. ⋆p<0.05, ⋆⋆p<0.01, ⋆⋆⋆p<0.001.