accumulation is enhanced in DKO livers during prolonged fasting.

<p>(A to E) Livers were isolated before (0 hour) and after fasting (24- and 48-hr). (A) Representative gross appearance of the liver. (B) Oil red O staining. Scale bar = 100 µm. (C) Triglyceride content in the liver (mg/mg protein). (D) Body weight (BW), % reduction in BW after fasting, liver weight (LW), ratio of LW relative to BW (LW/BW, mg/g). n = 11/group. (E) The expression of genes involved in FA uptake was determined using quantitative real-time PCR. (F and G) Mice received intravenous injections of ¹²⁵I-BMIPP (5 kBq) and ¹⁸F-FDG (100 kBq) via the lateral tail vein before (0-hr) and after fasting (24- and 48-hr). The animals were sacrificed at 2 hours after injection. Uptake of ¹²⁵I-BMIPP (F) and ¹⁸F-FDG (G) by the liver was quantified using a well-type gamma counter (n = 4). (H) Hepatocytes were isolated from WT and DKO livers. Uptake of ¹⁴C-palmitic acid (¹⁴C-PA) by hepatocytes was measured using a liquid scintillation counter. Note that uptake of ¹⁴C-PA was comparable between WT and FABP4/5 DKO hepatocytes. In addition, the uptake was proportional to the loading dose of ¹⁴C-PA. n = 6/group. Data are shown as the mean ± SE. WT vs. DKO. ⋆p<0.05, ⋆⋆p<0.01, ⋆⋆⋆p<0.001, ns = not significant.