Overexpression of KSRP limits axonal outgrowth in hippocampal neurons.

<p><b>A</b>. GFP-KSRP constructs cloned for KSRP overexpression studies. GFP-KSRP-KH 1–4 lacks a NLS, while the GFP-KSRP-ΔKH4 lacks both a NLS and the fourth KH domain. <b>B</b>. Rat hippocampal neurons transfected with KSRP constructs. Transfection conditions: a) GFP, b) KSRP, c) KSRP KH 1–4, d) KSRP ΔKH4. Scale bar is 100 µm. <b>C</b>. Quantitation of axonal outgrowth in KSRP transfected hippocampal neurons. Averaged axonal outgrowth from several transfection experiments is shown (mean +/− SEM). **, p<0.01; ***p<0.001 using a One way ANOVA with Tukey’s multiple comparison post-hoc tests (GFP n = 8, KSRP n = 11, KSRP KH 1–4 n = 6, KSRP ΔKH4 n = 10).