Time courses showing changes in the numbers of cells in the presence or absence of epidermal growth factor (EGF) or nerve growth factor (NGF).

<p>Time courses of three cell states were observed in experiments (circular dots), and fitted simulation results (lines) for the high serum (A–C), low serum (D–F), and serum free (G–I) conditions in the presence or absence of EGF or NGF. For each experiment, we counted to calculate the average number of cells for each day. Typical results of four independent experiments in each condition are shown. The parameters in <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1003320#pcbi.1003320.s008" target="_blank">Table S1</a> were applied to simulate a mathematical model. For each figure, lines denote the average number of proliferating (red), differentiated (blue), and dead (green) cells. Error bars denote confidential region fitted by exponential distribution. HS, horse serum; FBS, fetal bovine serum.