Changes in entropy with time.

<p>(A) Serum conditions used were 10% horse serum (HS) and fetal bovine serum (FBS) (black), HS and FBS (red), and BSA (blue). The definition of entropy is . The dots show the experimental results and the lines are the results of simulation. The maximum entropy value () was determined for when , and the middle entropy value () was determined for when and . The parameter values in <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1003320#pcbi.1003320.s008" target="_blank">Table S1</a> were used for the simulations. (B) Under serum-free conditions, the entropy was high, and the numbers of the three cell types on the dish were similar. Under low-serum conditions, levels of entropy were intermediate, and most of the cells were either proliferating or dead. Under high-serum conditions, most of the cells were proliferating.