Comparative analysis data of the qPCR amplification curves of BoR300 repeats and the single copy gene (<i>ace</i>), for the absolute estimation of BoR300 copies.

a,b<p>Intercept and slope of the linear regression lines (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0079393#pone-0079393-g006" target="_blank">Figure 6</a>).</p>c<p>The efficiency of the reaction was estimated by the equation: E = 10^−1/slope-1.</p>d<p>ΔCt value is obtained by comparing the y-axis intercepts derived from the two lines-of-best-fit of the target sequences BoR300 and <i>ace</i>.</p>e<p>According to the mean efficiency of the reactions (94.05%) the amplification factor F was 1.881.