E4orf4 causes p107 hyperphosphorylation and prevents binding of p107 to PP2A<sup>B55α</sup>.
- Melissa Z. Mui (485047)
- Michael Kucharski (485048)
- Marie-Joëlle Miron (485049)
- Woosuk Steve Hur (485050)
- Albert M. Berghuis (352046)
- Paola Blanchette (485051)
- Philip E. Branton (485052)
Publication date
November 2013
DOIAbstract
<p>Effects of E4orf4 on PP2A<sup>B55α</sup> substrate p107. (<b>A</b>) Whole cell extracts (WCE) prepared from cells transfected with HA-E4orf4 and FLAG-B55α and western blots performed using anti-p107, anti-FLAG, and anti-HA antibodies. (<b>B</b>) Co-immunoprecipitation experiments where wild type FLAG-B55α or mutant species (D340K, E91A/E93A/E94A/F343A, and D197K) were immunoprecipitated using anti-FLAG antibodies and western blots performed using anti-p107 antibodies. WCE shown for HA-E4orf4, FLAG-B55α species, and p107.</p
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