ERK affects CRE-luciferase reporter activity.

<p><i>A</i>, Western blot showing an increase in the nuclear localization of ppERK activation following 12 h of sleep deprivation in 5 day old <i>Cs</i> flies (40 heads/lane). <i>B</i>, Quantification of the Western blot in <b><i>A</i></b> revealed a statistically significant increase in ppERK/TotERK levels, students t-test, p = 0.032, n=8. Data are presented as mean ± SEM. <i>C</i>, Sleep profile of flies expressing a wild type version of <i>UAS-RSK</i>^<i>wt</i> displayed a decrease in sleep. <i>D</i>, Total sleep is significantly decreased in RSK over expressing flies. p = 0.0261; student’s t-test, n= 31-32/group. <i>E</i>, <i>elav/Y; CRE-Luc/UAS-ERK</i>^<i>SEM</i> flies (blue trace) have elevated total reporter activity compared to <i>elav/Y; CRE-Luc/+</i> controls (red trace); n=19-21/group. <i>F</i>, Sum of luciferase activity shown in <b><i>E</i></b>; students t-test, p =0.04). <i>G</i>, Flies that are heterozygous for the MAPK gene <i>rolled</i> (<i>CRE-Luc/Y; rl</i>^10A<i>/+</i>; blue trace) show significant reductions in reporter activity compared to controls (<i>CRE-Luc/Y; +/CyO</i>; red trace); n=16-18/group. <i>H</i>, Sum of luciferase activity shown in <b><i>G</i></b>; students t-test, p = 0.0006). <i>I</i>, SL327-fed <i>elav/Y; CRE-Luc/+</i> (black trace) show significant reduction in reporter activity compared to vehicle controls (red trace); n=48/group. <i>J</i>, Sum of luciferase activity shown in <b><i>I</i></b>; students t-test, p = 0.008).