The function of Mpc proteins in ROS balance and survival in stationary phase.

<p>A–B. The yeast strains overexpressing individual Mpc proteins described in Fig. 4 were grown in normal SD medium and subjected to a brief oxidative stress caused by hydrogen peroxide (A) or menadione (B). ROS was measured by the oxidation of dichlorodihydrofluorescein as described in “Experimental Procedures”. Fluorescence of the wild type before stress was arbitrarily set to 1. ROS levels were determined for three independent cultures in duplicate. Data presented are mean values +/− SD. Mpc1 and Mpc2 overexpressing strains have significantly increased ROS levels (p = 0.02 for panel A; p = 0.05 for panel B) as compared to wt according to the Students t-test (B). C. The survival in stationary phase in YPD medium of the indicated yeast strains was determined by plate assays after the indicated time.