Expression of <i>Csmd1</i> promoter-associated long non-coding RNA (<i>pas-lncRNA</i>) in mouse tissues.

<p>(A) Map of RNA sequencing reads aligned to the promoter region of <i>Csmd1.</i> Data from individual <i>Csmd1</i> KO (N = 4; red lines) and WT (N = 4; green lines) mice are shown. The novel <i>pas-lncRNA</i> RNA (black thick line) is expressed antisense to the <i>Csmd1</i> promoter sequence (transcript scale: 0–30 reads). (B) Heat map representing the relative expression level of <i>pas-lncRNA</i> and <i>Csmd1</i> mRNA in peripheral and CNS tissues of <i>Csmd1</i> KO and WT mice, respectively. Expression values of each transcript are calculated relative to their respective expression level in cortex of WT mice. <i>pas-lncRNA</i> expression was induced in the CNS but not in peripheral tissues of <i>Csmd1</i> KO mice. Fold change values (FC) and statistical significances are listed for each tissue. (C) Co-regulated expression of <i>Csmd1</i> and pas-lncRNA in the developing cortex and cerebellum (<i>Csmd1</i>:<i>pas-lncRNA</i> expression correlation coefficient, cortex: r² = 0.92). Y-axis represents relative expression level of RNA. X-axis indicates the postnatal day. Abbreviations: asterisk, t-test <i>P</i>-value<0.05; n.s.; not significant.