SDS-PAGE analysis of recombinant proMP2_Xa, proMP2, Spn27A and PPO1.

<p>The purified recombinant proMP2_Xa (0.7 µg), proMP2 (0.99 µg) and Spn27A (2.6 µg) were treated with SDS sample buffer containing β-mercaptoethanol and separated by 10% SDS-PAGE followed by coomassie brilliant blue staining. Recombinant PPO1 (1.3 µg) was separated by 7.5% SDS-PAGE. The sizes and positions of the molecular mass standards are indicated on the <i>right</i>.