BDNF-induced phosphorylation of ERK1/2 and AKT are enhanced by overexpression of SH2B1β.

<p>(A) PC12-GFP+TrkB or PC12-SH2B1β+TrkB cells were cultured in serum-free medium overnight before stimulation of 50 ng/ml BDNF for the indicated time points. Lysates were collected and equal amount of proteins was separated by SDS-PAGE and immunoblotted with anti-pERK1/2, ERK1/2, pAKT(S473), AKT, pPLCγ1(Y783), and PLCγ1 antibodies. Representative blots from three independent experiments are shown. (B) pERK1/2, pAKT(S473), and pPLCγ1(Y783) levels were normalized to total ERK1/2, AKT and PLCγ1, respectively. The relative pERK1/2, pAKT(S473) and pPLCγ1(Y783) levels for the 5 min time point of PC12-SH2B1β+TrkB cells were used as 1. The error bars represent S.E.M. from three independent experiments. (*: p<0.05, paired Student’s t-test)