Phosphorylated CREB decreased 48-N₂O anesthesia as well as 15 min after probe test.

<p>(A) The levels of pCREB in the Sev+N₂O group were lower than the Con group, but no differences of CREB levels were detected. (B) Relative levels of pCREB and CREB were quantified (<i>p</i> = 0.029 for pCREB, <i>p</i> = 0.923 for CREB). (C) The pCREB levels in the hippocampus of Sev+N₂O-MWM rats were significantly reduced compared to the Con-MWM rats, but CREB expression had no significant difference. (D) Relative levels of pCREB and CREB were quantified (<i>p</i> = 0.01 for pCREB, <i>p</i> = 0.58 for CREB). *<i>p</i><0.05, **<i>p</i><0.01.