Decreased nephrin expression.

<p>Western blot analysis of podocytes showing a down-regulation of nephrin with increasing concentrations (0-100 nM) of EVL and SRL after 48 h of incubation, respectively (A, B); expression of nephrin in the simultaneous analysis (20 nM) was lower in SRL than in EVL (C); α-tubulin served as loading control. Immunofluorescence staining of nephrin (D) and analysis (E) revealed less nephrin protein in SRL compared to EVL. Magnification 400×, scale bar represents 50 µm. EVL = Everolimus; SRL = Sirolimus; C_Ø = untreated control; C, Control = solvent ethanol control; PAN = puromycin aminonucleoside. ***<i>P</i> < 0.001, **<i>P</i> < 0.01.