Assay design and optimization.
- Jayme L. Dahlin (486120)
- Rondedrick Sinville (486121)
- Jonathan Solberg (486122)
- Hui Zhou (13228)
- Junhong Han (147191)
- Subhashree Francis (486123)
- Jessica M. Strasser (486124)
- Kristen John (381275)
- Derek J. Hook (486125)
- Michael A. Walters (486126)
- Zhiguo Zhang (147197)
Publication date
November 2013
DOIAbstract
<p>(A) Titration matrix of CoA and CPM in buffer-only conditions to determine the optimal assay levels of acetyl-CoA and CPM. (B) Titration matrix of CPM and acetyl-CoA in buffer-only conditions to verify acetyl-CoA and CPM do not form fluorescent adducts under HTS conditions. (C) Time-course study of CoA titrations with 20 µM CPM in buffer-only conditions to determine the optimal time for the reaction involving CoA and CPM. (D) HTS plate template. Arrows denote chosen HTS conditions.</p
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