CRF2-mediated dissociation of AJ leads to nuclear shuttling of p120ctn and Kaiso.

<p>(A) Confocal analysis of p120ctn location in HT-29 cells pretreated with cycloheximide before Ucn3 treatment. Scale bar, 10 µm. (B) Immunoblot analysis of p120ctn and Kaiso protein expression in nuclear extracts from HT-29 cells treated or not with Ucn3 (expression was normalized to histones). (C) Effects of Src and MEK inhibitors (PP2 and U0126: 10 µM, 1 hr) on p120ctn and Kaiso protein expression in nuclear extracts from HT-29 treated or not with Ucn3. Expression was normalized to histones. (D) Confocal analysis of cellular distribution of Kaiso in HT-29 cells. Nuclei were stained with Topro3. Cells negative for nuclei labeling of Kaiso were circumscribed by a white dotted line. Scale bar, 60 µm.