CRF2 signaling disrupts AJ.

<p>(A) Immunoblot analysis of Src-P^Tyr418 and Src total expression in HT-29 cells following the time course of Ucn3 treatment. (B) Confocal analysis of E-cadherin distribution in HT-29 cells pretreated with cycloheximide before Ucn3 treatment. Scale bar, 15 µm. (C) Effect of PP2 (10 µM, 1 hr) on E-cadherin distribution in HT-29 cells treated or not with Ucn3, analyzed by epifluorescence microscopy. Scale bar, 20 µM. (D) Effect of CRF2 antagonist (A2b, 8 nM, 24 hrs) on E-cadherin expression in SW620 cells. Quantification was performed from immunoblots and normalized to actin. (E) E-cadherin distribution in SW620 cells pretreated or not with A2b and further treated or not with Ucn3. Analyzed were performed by epifluorescence microscopy. Scale bar, 10 µM.