TGF-β1/H₂O₂/HOCl facilitates invasive capability of HCC cells.

<p>(<b>A</b>) Tumor cells were cultured in presence of TGF-β1 or T/H/H (TGF-β1, 5 ng/ml, H₂O₂, 100 µM, HOCl, 50 µM) for the indicated time, and then used for Matrigel invasion assay. (<b>B</b>) After 10-d culture in absence or presence of TGF-β1, H₂O₂ and HOCl, tumor cells were used for Matrigel invasion assay. (<b>C</b>) Tumor cells were treated for 10 days with H₂O₂/HOCl, TGF-β1, or T/H/H, and then incubated in presence of matrigel for 5 h. The cells with highly polymerized actin were visualized by staining with rhodamine-phalloidin (left). Their percentage in total cells was calculated (right). (<b>D</b>) Tumor cells were treated as described in C, and then cultured in presence of matrigel for 48 h. The MMP-2 and MMP-9 in supernatants were detected by zymography assay. The fold difference of active MMP-2 and MMP-9 was calculated after densitometric analysis of the gel. <i>P</i> values, *<i>P</i> < 0.05, **<i>P</i> < 0.01.