Overexpression of G3BP1 inhibits CVB3 replication.

<p>HeLa cells were transiently transfected with pEGFP-G3BP1 for 24 hrs, followed by CVB3 infection at an MOI of 1 for 16 hrs. (A) Western blotting (left) and confocal microscopy (right) were performed to examine protein level and intracellular distribution of GFP-G3BP1. (B) Western Blotting was performed to assess protein expression of VP1 and β-actin after CVB3 infection. (C) Densitometric analysis using ImageJ was performed on VP1 intensities relative to β-actin intensities of three independent experiments from (B). The value of sham was arbitrarily set as 1. (D) Quantitative RT-PCR was performed to examine viral transcript levels using primers specific to the IRES promoter of the CVB3 RNA transcript. Data is presented as transcript copy number relative to its empty vector control. The value of the empty vector control was arbitrarily set as 1. (E) Plaque assay was performed to examine the effect of G3BP1 overexpression on viral replication and results are presented as relative PFU/ml. The value of the empty vector control was arbitrarily set as 1. Results are presented as mean ± SD (n = 3), #p<0.001, *p<0.01.