Time-course of actin distribution around the animal pole.

<p>Actin distribution (A) before and (B) at the onset of, and (C, D, E) after the bulge formation. Actin fluorescence distribution that had been analyzed previously <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0079389#pone.0079389-Hamaguchi1" target="_blank">[10]</a> is shown in pseudocolor on the 3D cell surface whose image is restricted to the surface near the animal pole, at the height of 20 µm except for the bulge. For comparison with the data of the relative tension at the surface in Fig. 5, the fluorescence distribution is normalized by the ratio of actin fluorescent intensity around the animal pole to that at the surface at a vertical distance of 20 µm below the animal pole. A color scale indicating the normalized actin fluorescence from 0 to 2.5 is shown at the bottom right of the figure. Scal bar, 50 µm.