The ultrastructural changes of the rats’ ovarian tissues in the control, PCOS, and PCOS EA groups.

<p>The ultrastructural changes of the rats’ ovarian tissues were observed by transmission electron microscopy, as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0079382#s2" target="_blank">Materials and Methods</a> section. A) A representative rat’s ovarian tissue section from the control group, which had normal appearance (A×10000). B) A representative rat’s ovarian tissue section from the PCOS group, which showed a reduced number of organelles, condensation and margination of the nuclear chromatin, mitochondrial swelling, broken or even disappeared mitochondrial cristae, and expanded endoplasmic reticulum (B×10000). C) A representative rat’s ovarian tissue section from the PCOS EA group, which showed increased mitochondria in granular cells, many cristae (with some destroyed) in part of the mitochondria, condensation and margination of nuclear chromatin (C×10000). M: mitochondria, N: nucleus, RER: rough endoplasmic reticulum, NC: nuclear chromatin, MC: mitochondrial cristae. ☆ indicated the changes of nucleus, ★ indicated the changes of rough endoplasmic reticulum, △: indicated the changes of nuclear chromatin, ▴ indicated the changes of mitochondria and mitochondrial cristae. Scale bar, 1 µm.