ILK protects against DOX-induced apoptosis.

<p><b>A</b>, (top panel) Immunohistochemical staining for TUNEL-positive apoptotic nuclei in Hu iPSC-CMs transduced with ad-ILK<i>WT</i>, ad-ILK<i>R211A</i>, ad-GFP and in non-transduced cells following exposure to 1µM DOX for 24 hours. Scale bar, 100 µm. Arrows point to TUNEL-positive apoptotic nuclei. (bottom panel) Quantitative analysis showing the percentage of TUNEL-positive cell in Hu iPS-CMs after adenoviral infection and exposure to DOX. Bar graphs represent mean values ±SD, n=10 (random fields). * <i>P</i> < 0.01, ** <i>P</i> <0.02 by student <i>t</i> test. <b>B</b>, Western blots indicating the expression levels of ILK (on the left) and quantitative analysis showing the percentage of TUNEL-positive cell (on the right) in Hu iPS-CMs treated with ILK siRNA (siILK) or scrambled siRNA (siControl) following exposure to 1µM DOX for 24 hours (+DOX). Bar graphs represent mean values ±SD, n=10 (random fields). * <i>P</i> < 0.01 by student <i>t</i> test. <b>C</b>, Immunoblot showing the expression levels of ILK, Hsp70, Hsc70 and Hsp90 in Hu iPS-CM transduced with ILK siRNA (siILK) or scrambled siRNA (siC) following exposure to 1µM DOX for 24 hours (+DOX) as compared to DOX untreated cells (-DOX).