Effects of FGF2, BDNF, GDNF and VEGF in ADP proliferation.

<p>A. Detection of mRNA of TrkB, GDNFR, FGFR1, Flk1 and Flt1 mRNA of TrkB, GDNFR and FGFR1 was detected, but mRNA of Flk1 and Flt1, both are receptors of VEGF, were not detected. GAPDH was used as an internal control. B. Effect of FGF2 on ADP proliferation FGF2 increased ADP proliferation in a dose-dependent manner. BrdU was added into medium 1 day after FGF2 treatment. BrdU immunocytochemistry was perfomed 24 hours after BrdU addition. Data are shown as the means ± SEM. Statistical analysis was performed using one-way ANOVA and Dunnet’s post hoc test. Significance was defined as <i>p</i><0.05, compared with 0 ng/ml. Scale bar = 120 <i>μ</i>m. C. Effect of BDNF on ADP proliferation BDNF had no effect on ADP proliferation. BrdU was added into medium 1 day after BDNF treatment. BrdU immunocytochemistry was perfomed 24 hours after BrdU addition. Data are shown as the means ± SEM. Statistical analysis was performed using one-way ANOVA and Dunnet’s post hoc test. Significance was defined as <i>p</i><0.05, compared with 0 ng/ml. D. Effect of GDNF on ADP proliferation GDNF had no effect on ADP proliferation. BrdU was added into medium 1 day after GDNF treatment. BrdU immunocytochemistry was perfomed 24 hours after BrdU addition. Data are shown as the means ± SEM. Statistical analysis was performed using one-way ANOVA and Dunnet’s post hoc test. Significance was defined as <i>p</i><0.05, compared with 0 ng/ml.