Relative contribution of apoptosis and autophagy to resveratrol enhanced UVB-induced HaCaT cells death.

<p>(A) HaCaT cells were pretreated for 24 hours with 25 µM resveratrol followed by 2 hours in the presence or absence of pan caspase inhibitor Z-VAD (50 µM) prior to irradiation with UVB (30 mJ/cm²). Cell count was performed after additional 24 hours of culture in standard medium. Graphs report mean values ± standard deviation of three independent measurements for each experimental point. RSV: resveratrol; * p<0.05. (B) “Total” represents the difference of cell number between UVB-irradiated HaCaT cells and resveratrol pre-treated and UVB-irradiated HaCaT cells; “Apoptotic” represent the difference of cell number between resveratrol/Z-VAD pre-treated/UVB-irradiated HaCaT cells and resveratrol pre-treated and UVB-irradiated HaCaT cells; Both “Total” and “Apoptotic” have been reported as percentage of “Total”(100% and 45% respectively). “Non-apoptotic” (55%) represent the difference of the percentages between “Total” and “Apoptotic”.