Functional role of miR-96 in vitro.

<p>LNCaP and DU145 cells were transfected with 10 nM pre-miR-96, anti-miR-96, pre-miR-NC #1 or combination of pre-miR-96 and anti-miR-96. The effect in (A) LNCaP and (B) DU145 on cell proliferation under serum starvation was measured by MTT assay over three days. All data were normalized on proliferation of control cultures on day 1 and are shown as mean (±SD) of three independent assays. *P <0.05, Two-way ANOVA. Cell cycle transition was measured by PI staining in transfected (C) LNCaP and (D) DU145 cells. Cells were serum starved one day after transfection for another 24 hrs and subsequently fixed and stained. Black, G1-phase; light grey, S-phase; dark grey, G2/M-phase. Data are shown as mean of three independent assays. Apoptosis in CPT-treated (E) LNCaP and (F) DU145 cells. 24 h after transfection cells were treated with 10 µM CPT for 24 h. Cells were stained with Annexin V-FITC and PI. Fraction of early (black bars) and late (white bars) apoptotic cells was measured by flow cytometry. Data are shown as mean (+SD) of three independent assays. *P <0.05; Bonferroni post-test (P < 0.001, Two-way ANOVA).