Analysis of the DNA damage tolerance pathway in <i>mec1</i><i>Δ</i> and <i>rad53</i><i>Δ</i> checkpoint kinase mutants.

<p>Serial dilutions (ten-fold) of indicated strains (see below) plated on YPAD plates with MMS and without the alkylating chemical. (A) wild-type, <i>rad53Δ sml1Δ</i>, <i>mms2Δ</i>, <i>rev3Δ</i>, <i>mms2Δ rad53Δ sml1Δ</i> and <i>rev3Δ rad53Δ sml1Δ</i>. (B) wild-type, <i>mec1Δ sml1Δ</i>, <i>mms2Δ</i>, <i>rev3Δ</i>, <i>mms2Δ mec1Δ sml1Δ</i> and <i>rev3Δ mec1Δ sml1Δ</i>. Viable plot graphs in (A) and (B) show the viability analysis of wild-type, <i>rad53Δ sml1Δ and mec1Δ sml1Δ</i> strains in <i>mms2Δ</i> or <i>rev3Δ</i> backgrounds (as indicated).