Further characterization of cultured striatal cells.

<p>(A) A representative wild-type cell was positive for GAD65 and for MAP2, indicating that this cell is a GABAergic neuron. (B) A representative field in the wild-type striatal culture contained GAD65-positive structures such as nerve terminals and soma, but lacked VGLUT1-positive glutamatergic structures. (C) In comparison, wild-type hippocampal neurons in culture contain many nerve terminals that are positive for VGLUT1 and GAD65. In each row, a cell was imaged with DIC optics (left), two fluorescence channels, and was shown in a merged fluorescence image (right). The scale bar in the top-left panel applies to all panels. Similar results were obtained from heterozygous neurons (data not shown).