Spironolactone ameliorates bleomycin-induced pulmonary fibrosis.

<p>Figures A to D, Masson’ trichrome staining of lung tissue from day 21. E to H show the magnified fields as indicated by black frames in A to D, respectively. Figures I to L show the immunofluorescent staining of α-SMA (FITC-labled, green; nuclei were counterstained by DAPI). Figures M to Q show fibrosis index, total lung hydroxyproline content, collagen type I and collagen type III mRNA expression and α-SMA positive area comparisions in lung tissue from day 21, respectively. Abbreviations: α-SMA, α smooth muscle actin; BLM, bleomycin; Hyp, hydroxyproline; Col I, collagen type I; Col III, collagen type III; NS, normal saline; SP, spironolactone. *P<0.05; **P<0.01 (n = 5 to 7).