Characterization of bioluminescent strains <i>in</i><i>vitro</i>.

<div><p>Serial dilutions of bioluminescent strains transformed with either plasmid (H347pTHLK) or integrated construct (H347::lux) were imaged in a 96 well plate (n= 3 separate cultures, Linear regression, r²&gt;0.95, * <i>p</i> &lt;0.05) using an IVIS 100 system (Perkin Elmer) to relate luminescence to bacterial abundance. Bacteria were quantified after plating onto agar (A). </p> <p>H347pTHLK (B) and H347::lux (C) were serially passaged <i>in </i><i>vitro</i> without antibiotic over 7 days; Samples of each culture were plated onto kanamycin solid medium or onto non-antibiotic media and demonstrated a failure of H347pTHLK to grow when cultured on antibiotic containing agar after 72h, consistent with loss of the plasmid. n=3 separate cultures, Repeated measures Two way ANOVA with Bonferroni correction, * <i>p</i> &lt;0.05). Error Bars indicate median and range.</p>