DNA methylation and allelic expression analyses of <i>Dnmt1o^mat−/−</i> female placentae.

<p>(A) Sex-specific restriction landmark genomic scanning (RLGS) analysis of single-copy gene DNA methylation in <i>Dnmt1o^mat−/−</i> 9.5dpc placentae. Bars indicate the number of spots showing hypo- and hypermethylation changes in each <i>Dnmt1o^mat−/−</i> placenta (P). (B) Evidence of relaxation of paternal imprinted XCI in female <i>Dnmt1o^mat−/−</i>offspring. Allele specific expression assay (RT-PCR) on X chromosome linked genes. The RNA used was extracted from the visceral endoderm layer of yolk sacs from 9.5dpc <i>Dnmt1o^mat−/−</i> and wild-type control (<i>Dnmt1o^mat+/+</i>) extraembryonic tissues. Maternal (129(M)) and paternal (Cast(P)) control fragments were derived from 129/sv and <i>Mus musculus castaneus</i> embryo RNA respectively. The 129× Cast control fragments were generated from F1 hybrid embryo RNA (129/sv × <i>Mus musculus castaneus</i>). Schematic on the right shows approximate gene positions (Mb) on the X chromosome.