Kinetics of RdRP activity of MBP-TNV-D p60 and effects of S1 nuclease treatment on radiolabelled RNA products.

<p>Panel (a) shows representative denaturing gel analysis of radiolabelled RNA products synthesised at 25°C (RT) by <i>in </i><i>vitro</i> transcription using satC- RNA as the template over a 16 h time course. The data is also shown in graphical form quantified by AIDA following phosphorimager analysis. Panel (b) shows the effects of S1 nuclease treatment on radiolabelled RNA products synthesised at 25°C. Products synthesised following 1, 2 or 3 h incubation were untreated (lanes 3, 5 and 7 respectively) or treated with S1 nuclease (lanes 4, 6 and 8 respectively). Radiolabelled products were stained with ethidium bromide in the left hand panel which also shows the template satC- RNA used in these reactions, untreated (lane 1) or treated (lane 2) with S1 nuclease, which is arrowed. Phosphorimaging of the gel is shown in the right hand panel.