Effect of mutations to MBP-p60 on the synthesis of STNV-C RNA.

<p>The upper tier in panel (a) shows 10% SDS-PAGE analysis of the expressed proteins, the lower tier shows 5% denaturing polyacrylamide-8 M urea gel analysis of radio-labelled STNV-C dsRNA. Individual mutations are shown above the respective lanes. Radioactivity was quantified by AIDA and expressed as a percentage of the radio-labelled dsRNA produced by wt MBP-p60 in four parallel assays and the data presented as histograms in panel (b).