EAC was induced by injecting female SWXJ mice sc with 0.2 ml emulsion and 0.1 ml DDDH₂O containing lyophilate from SWXJ mice with 0.1 ml of complete Freund’s adjuvant (CFA) containing 400 µg of <i>Mycobacterium tuberculosis</i> H37R1.

<p>Changes in systemic CXCL9, CXCL10 and CXCL11 levels in EAC mice and CXCL10 in naive mice were measured 3 month after EAC induction (panel A). After 3 months of EAC induction, control or anti-CXCL10 Ab solutions were administered every 2 days for eight weeks. At the end of experiments, serum levels of CXCL9 (▪), CXCL10 (♦), CXCL11 (▾) in experimental and naïve mice (○) (panel B) were determined by ELISA assays. The data presented are the mean concentrations in each group. (A) Asterisks indicate statistically significant differences, i.e., <i>p</i><0.01 (*), between naïve and CXCL10 groups. (B) <i>p</i><0.01 (*), between CXCL10 level of EAC-induced control and anti-CXCL10 Ab-treated groups.