Endocytotic activity of neutrophil-DC hybrids emerging in IFNγ-treated animals.

<p>C57BL/6 mice (3 animals/panel) received i.p. injections of recombinant IFNγ or PBS alone immediately before and 24 h after thioglycollate treatment. PEC samples harvested on Day 2 were incubated for 60 min at 37°C with live <i>E.coli</i> expressing GFP and then examined for GFP fluorescence signals within the Ly6G⁺/CD11c⁻ neutrophil, Ly6G⁺/CD11c⁺ hybrid, and Ly6G^-/CD11c⁺ conventional DC populations. (A) Data shown are representative histograms after incubation in the presence (red) or absence (blue) of GFP-<i>E</i>.<i>coli</i>. (B) Data shown are the means ± SD MFI values from three animals. Asterisks indicate statistically significant differences (*P < 0.05, **P < 0.01; ***P < 0.001).