Superposition of ¹H-¹⁵N-TROSY spectra of E.coli-YLOV and its reconstituted variants.

<p>(A) ¹H-¹⁵N-TROSY spectra of E.coli-YLOV (black) and FMN-YLOV (red). All sequentially assigned resonances of E.coli-YLOV are labeled with residue type and sequence position. (B) ¹H-¹⁵N-TROSY spectra of FMN-YLOV (black) and RF-YLOV (red). Sequentially assigned resonances of FMN-YLOV possessing different chemical shifts in RF-YLOV are labeled. Note that the signal from T⁸⁰ is not shifted indicating that the chemical environment of this residue is the same in both proteins. (C) ¹H-¹⁵N-TROSY spectra of FMN-YLOV (black) and FAD-YLOV (red). All both evaluable and sequentially assigned resonances of FMN-YLOV that are either changed in terms of intensity, shifted or unaffected in FAD-YLOV are labeled.The insets in A and B show a close up of the region of chemical shifts involving R⁶³ and R⁷⁹. All spectra were recorded from uniformly ¹⁵N-labeled samples.